SMART BioSyM Seminar 12 Oct 2015
Event Date: 12 Oct 2015 08:00 AM - 12 Oct 2015 09:00 AM
Event Venue: CREATE Enterprise Wing Level 5, Perseverance Rooms 1 & 2
Volume holographic imaging system (VHIS) has recently been demonstrated for imaging in vivo three-dimensional biopsy without mechanical or optical axial scanning. Compared to confocal microscopes, VHIS has the advantage of taking wide field images at multiple depths with optical sectioning ability in a single exposure. Thus, it can enable fast wide field imaging with good axial resolution. Due to the volume grating nature of the hologram, VHIS has shift-variant and wavelength-dependent point spread function. This has led to a challenging task to analyze and predict the performance of the VHIS.
Based on a weak diffraction assumption, we can model the point spread function of the VHIS using Fourier optics method. Here we present a simulation model based on this method to investigate the point spread function and image formation of VHIS. We are able to simulate the whole field intensity image on the detector plane given any random field input. We can also simulate the effect of light source spectrum to the image. With this proposed simulation model, we can well characterize the performance of a VHIS. We will also show an application using this model: with a good knowledge about the system’s point spread function, we can use optimization method such as TwIST to compressively sense sparse signals in noisy background, such as imaging in deep tissue where scattering of light cause strong noise.